University of Connecticut University of UC Title Fallback Connecticut

Gel Permeation Chromatography

* GPC set-up may change according to need.  Please consult with lab manager for current set-up details.

 

Waters GPC-1 (1515 HPLC Pump & Waters 717Plus Autoinjector)*
System1 Mobile phase: Tetrahydrofuran (THF)
Column set: Jordi Gel fluorinated DVB columns (1-100K, 2-10K & 1-500Å). Lower molecular weight column set available.
Injection volume: 10µl – 200µl.  Optional 2400 µl sample holding loop available.
Autosampler: Temperature range: 4-40⁰C
Column oven: Four column capacity, temperature range up to 80⁰CSample carousels: 48 vials (4-ml) & 96 vials (1-ml)
Standards: Polystyrene & polymethylmethacrylate
Detectors:

  • Waters 2414 Refractive Index Detector (RI): Maximum temperature 50⁰C
  • Waters 2487 Dual Absorbance Detector (UV/VIS): Wavelength range 190-700nm. Monitors two wavelengths simultaneously
  • Varian 380-LC Evaporative Light Scattering Detector (ELSD): Nebulising gas nitrogen. Temperature range ambient to 90⁰C (nebuliser) & 120⁰C (evaporator)

Application: Provides the molecular weight distribution of polymers fully soluble in tetrahyrofuran

 

Agilent 1260 Infinity GPC (System II)*
System2 Mobile phase: Toluene
Injection volume: Up to 100µl
Sample carousels: 100 vial (1.5-ml)
Column set: Two mixed bed PL columns
Column oven: Three column capacity, temperature range 10-60⁰C
Standards: Polystyrene & polymethylmethacrylate
Detectors:

  • Refractive Index Detector (RI): Temperature range ambient plus 5⁰C to 55⁰C
  • Variable wavelength Detector: Wavelength range 190-600nm.

Application: Provides the molecular weight distribution of polymers fully soluble in toluene

 

Waters GPC-2 (Waters 515 HPLC pump & Waters 717 Autoinjector)*
System3 Mobile phase: Dimethylacetamide (DMAC), methylpyrrolidinone (NMP) or dimethylformamide (DMF)
Column set: Two mixed bed Jordi Gel DVB columns
Injection volume: 10µl – 200µl. Optional 2400 µl sample holding loop available
Autosampler temperature range: ambient to 40⁰C
Column oven: Four column capacity, temperature range up to 80⁰C
Sample carousels: 48 vial (4-ml) & 96 vial (1-ml)
Standards: Polystyrene & polymethylmethacrylate
Detector:

  • Waters 2414 Refractive Index Detector (RI). Maximum temperature 50⁰C

Application:  Provides the molecular weight distribution of polymers fully soluble in DMAC, NMP or DMF

 

Kayeness D4001 Melt Index Tester

MeltIndexer Sample charge: Up to 6 grams
Piston weight: 1.05, 1.2, 2.16 & 5 kg
Temperature: Up to 300⁰CApplication: Measures ease of flow of a thermoplastic polymer melt. Indirect measure of molecular weight.