University of Connecticut University of UC Title Fallback Connecticut

James L. Cole

ColeJamesLJames L. Cole

Department of Molecular & Cell Biology
91 North Eagleville Road
Storrs, CT 06269-3125
Phone: (860) 486-4333
Email: Web:


  • Postdoctoral Fellow: Bioinorganic Chemistry, Department of Chemistry, Stanford University, and Stanford, CA. 1991
  • Ph.D. in Biophysical Chemistry, Department of Chemistry, University of California, Berkeley, CA. 1987
  • Sc.B. in Biochemistry, Brown University, Providence, RI. 1981


Research Focus

  • Characterization of macromolecular interactions using analytical ultracentrifugation and related biophysical techniques
  • Double-stranded RNA activated protein kinase (PKR)
  • Interferon antiviral defense pathway
  • TRP RNA-binding attenuation protein (TRAP)


Current Publications

Eric Anderson, Willythssa S. Pierre-Louis, C. Jason Wong, Jeffrey W. Lary and James L. Cole (2011) “Heparin Activates PKR by Inducing Dimerization” J. Mol. Biol., doi:0.1016/j.jmb.2011.09.025.

Cole, J.L, Correia, J.J. and Stafford, W.F. (2011) “The Use of Analytical Sedimentation Velocity to Extract Thermodynamic Linkage Biophys.” Chem., in press: doi:10.1016/j.bpc.2011.05.014.

R. Bryk, K. Wu, B.C. Raimundo, P.E. Boardman, P. Chao, G.L. Conn, E. Anderson, J.L. Cole, N.P. Duffy, C.Nathan and J.H. Griffin (2011) “Identification of New Inhibitors of Protein Kinase R Guided by Statistical Modeling.” Biorganic and Medicinal Chemistry Letters in press: doi:10.1016/j.bmcl.2011.04.149.

Andy J. Wowor, Dongmei Yu, Debra A. Kendall and James L. Cole (2011) “Energetics of SecA Dimerization.” J. Mol. Biol., in press: doi:10.1016/j.jmb.2011.02.006.

C. Jason Wong, Katherine Launer-Felty and James L. Cole (2011) “Analysis of PKR-RNA Interactions by Sedimentation Velocity.” Methods in Enzymology 488, 59-79.

Cole, James L. (2010) “Analysis of PKR Activation Using Analytical Ultracentrifugation.” Macromol. Biosci. 10, 703-710.
Launer-Felty, K., Wong, C.J., Wahid, A.M., Conn, G.L. and Cole, J.L. (2010) “Magnesium-Dependent Interaction of PKR With Adenovirus VAI RNA.” J. Mol. Biol. 402, 638-644.

Anderson, E., Quartararo, C., Brown, R.S., Shi, Y., Yao, X. and Cole, J.L (2010) “Analysis of Monomeric and Dimeric Phosphorylated Forms of Protein Kinase R.” Biochemistry 49, 1217-1225.

Zhang, N., Wang, Q. Ehlinger, A., Randles, L., Lary, J. W., Kang, Y., Haririnia, A., Storaska, A. J., Cole, J. L., Fushman, D., Walters, K. J. (2009) “Structure of the S5a:K48-Linked Diubiquitin Complex and Its Interactions With Rpn13.” Mol. Cell 35, 280-290.

VanOudenhove, J., Anderson, E., Krueger, S. and Cole, J.L. (2009) “Analysis of PKR Structure by Small Angle Scattering.” J. Mol. Biol. 387, 910-920.

Heinicke, L.A., Wong, C.J., Lary, J., Nallagatla, S.R., Deigelman-Parente, A., Zheng, X., Cole, J.L. and Bevilacqua, P.C. (2009) “RNA Dimerization Promotes PKR Dimerization and Activation.” J. Mol. Biol. 390, 319-338.