Molecular Analysis – Thermo Fisher Nicolet Magna 560

ThermoFisher Nicolet Magna 560

Available Methods and Accessories

  • Specac Quest Attenuated Total Reflectance (ATR) (diamond or germanium crystals)
  • PIKE Technologies Grazing Angle (GA)
  • Equipment for grinding & pelletizing samples
  • 265k reference spectra from Wiley Spectral Libraries

Sample Requirements

Attenuated Total Reflectance Mode

  • Non-aqueous liquid or solid
  • Must cover 2mm2 crystal

Grazing Angle Mode

  • Extremely thin layers on a reflective substrate
  • Substrate 2 x 1.5 cm2 or larger

Transmission Mode

  • Thin films 1 cm2 or larger
  • Powder 1-2 mg
  • <10µL for liquids
  • Must be non-aqueous

Summary of Technique

A sample is illuminated with infrared light, the absorption of which causes molecular vibrations in the sample. These vibrations are characteristic of functional groups in the sample. A representative spectrum is shown to the left with some of the evident functional groups denoted. FTIR is not able to determine connectivity of functional groups.

Information Provided & Detection Limits

FTIR-ATR is capable of rapidly determining the major organic chemical species present in a sample, with a detection limit of 1-5% by weight. FTIR-GA is used for analyzing molecular layers on a reflective surface. FTIR-Transmission can provide a greater signal-to-noise ratio in some cases than attenuated total reflectance.

Lab Location and Contact Information

Lab Location: Science 1, G20
Lab Manager: Capri Price