Liquid Chromatography – Waters GPC-1 (1515 HPLC Pump and Waters 717Plus Autoinjector)


Available Methods and Accessories

  • 3 Solvents available: Tetrahydrofuran (THF), Water, N,N-Dimethylacetamide (DMAC)
  • UV, RI, ELSD detection

Sample Requirements

Samples should be dissolved in the appropriate solvent and filtered (0.45 micron) prior to analysis. Sample concentration should be approximately 1 – 5 mg/mL for polymers <100 kDa, 0.7 – 1.5 mg/mL for 100 – 500 kDa, and <0.7 mg/mL for >500 kDa.

Summary of Technique

Gel permeation chromatography utilizes the stationary phase pore volume to separate molecules of differing molecular weights. A larger molecule that’s unable to reside in the pore will spend more time in the mobile phase while the smaller molecules reside more in the stationary phase. Elution volumes are calibrated against molecular weight standards, polystyrene or poly(methylmethacrylate).

Information Provided & Detection Limits

Provides information on molecular weight distributions of polymers. Detection limit depends on the detector used.

Lab Location and Contact Information

Lab Location: Chromatography Lab
Lab Manager: Nicholas Eddy